Journal: Scientific Reports

Article Title: Ablation of TRPC3 disrupts Ca 2+ signaling in salivary ductal cells and promotes sialolithiasis

doi: 10.1038/s41598-023-32602-8

Figure Lengend Snippet: Calcification due to TRPC3 ablation upregulates oxidative stress and apoptosis in SMG. Densitometric analysis of ( A–C ) RT-PCR and/or ( D ) protein blot expressions for oxidative stress markers (CHOP, NOX4, FMO2, GPX3, GPX6, m18S, and SCD1) or apoptotic markers (BAX1/BCL2, Cas3, and Cas12) in WT and TRPC3 -/- (KO) mice SMG tissues were performed using ImageJ. GAPDH and β-actin were utilized as internal control for RT-PCR and immunoblotting, respectively. Each RT-PCR and immunoblotting experiment was performed from n = 3 mice. Full length blots are indicated in the Supplementary Materials. ( A–D ) Bar diagrams of quantitated RT-PCR or western blots depicted in mean + SEM. * P < 0.05; ** P < 0.01. ( E ) in situ apoptosis was detected in mice WT and KO SMG tissues.

Article Snippet: In situ apoptosis in mice tissue sections were performed using TACS•XL DAB in situ Apoptosis Detection Kit (Trevigen, Gaithersburg, MD, USA), as per manufacturer’s directions and our previously published method on mice kidney tissue section .

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Western Blot, In Situ